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KKU medical research published (kku news 465) |
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Written by phitsanu
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KKU medical research published
Research work and results/ short report of the Faculty of Medicine, KKU
A Short Report on “Molecular Identification of Trichinella papuae from a Thai patient with imported Trichinellosis” has been publicly announced, according to the research project head Prof. Piewphan Maleewong.
She has conducted this research and analyzed the base order of mitochondrial RNA of the Trichinella sp.
Parasites using a biopsy method from a sample of meat the patient had eaten. Eating infected wild boar meat is a common cause of the disease.
The laborers became infected while working in Malaysia with symptoms of swelling and inflamed at muscles.
The research found that the meat sample, which was dyed with Hematoxylin & Eosin, was infected with the parasite Trichinella spp. and had non- encysted larvae that could not be separated for microscope observation.
Therefore, the researchers have designed the specific primer by using the gene information on mitochondrial RNA of the Trichinella sp. parasite and then increasing their numbers by using the PCR as well as DNA sequencing methods.
From the analysis, it was found that it is the embryo of the parasite of Trichinella papuae (GenBank accession no. EF517130).
This research work is the first report of its kind that has found the infection of T.papuae among the Thai workers who had returned from working abroad. In general,Trichinella found most widely is T. spiralis in form of the encysted larvae.
This research is a model for further application of knowledge on bio- molecules that separate into concrete kinds of the Trichinella parasite, offering a new body of knowledge that could be used as a reference and in teaching and learning about the diseases, its diagnosis and treatment.
This research increased knowledge of the epidemiology of parasites and about the possibility of Trichinella papuae in wild animals in Thailand and in some Southeast Asian countries and its possibility of spreading.
This research model, therefore could be further studied about epidemiology of parasites, remarked Prof. Piewphan.
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